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              ScienCellGK039人類先天免疫反應(yīng)qPCR檢測(cè)試劑盒
              更新時(shí)間:2023-01-03   點(diǎn)擊次數(shù):625次

              GK039 ScienCell 人類先天免疫反應(yīng)qPCR檢測(cè)試劑盒,Human Innate Immune Response qPCR Array Kit

              ScienCell 研究實(shí)驗(yàn)室(ScienCell)是一家生物技術(shù)公司,其使命是實(shí)驗(yàn)和治療用細(xì)胞和細(xì)胞相關(guān)產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動(dòng)物細(xì)胞,細(xì)胞培養(yǎng)基和試劑,培養(yǎng)基添加因子,細(xì)胞來(lái)源的RNAcDNA和蛋白質(zhì)。ScienCell的科學(xué)家們以改善生活質(zhì)量,實(shí)現(xiàn)細(xì)胞的能力,打擊人類退化性疾病為目的研究和開(kāi)發(fā)各種細(xì)胞治療策略。ScienCell總部設(shè)在美國(guó)加利福尼亞州圣地亞哥,公司成立于1999年。

              北京諾博萊德作為ScienCell品牌的經(jīng)銷商,為客戶提供,正規(guī)進(jìn)口,貨期穩(wěn)定,折扣好的服務(wù)標(biāo)準(zhǔn)。

              GK039 ScienCell人類先天免疫反應(yīng)qPCR檢測(cè)試劑盒,Human Innate Immune Response qPCR Array Kit,產(chǎn)品簡(jiǎn)介:

              產(chǎn)品品牌: ScienCell

              產(chǎn)品貨號(hào):GK039

              產(chǎn)品名稱:GK039 ScienCell人類先天免疫反應(yīng)qPCR檢測(cè)試劑盒,Human Innate Immune Response qPCR Array Kit

              產(chǎn)品規(guī)格:One 96-well plate, one 20ul reaction per well


              GK039 ScienCell人類先天免疫反應(yīng)qPCR檢測(cè)試劑盒,Human Innate Immune Response qPCR Array Kit 產(chǎn)品說(shuō)明:

              ScienCellGeneQuery™人類先天免疫反應(yīng)qPCR陣列試劑盒(GQH-IIR)有助于人類先天免疫系統(tǒng)中關(guān)鍵基因的基因表達(dá)譜分析。 先天免疫反應(yīng)在微生物感染或異物出現(xiàn)時(shí)被激活,包括補(bǔ)體系統(tǒng)和適應(yīng)性免疫系統(tǒng)的激活。 一個(gè)單獨(dú)的陣列可用于分析適應(yīng)性免疫反應(yīng)。 如何根據(jù)功能對(duì)所包含的基因進(jìn)行分組的簡(jiǎn)要示例如下所示:

              細(xì)菌反應(yīng): TLR1 TLR5 NOD1 LY96 TRAM1

              病毒反應(yīng): TLR3 DDX58 IFIH1 MYD88 IRAK4

              自我識(shí)別: TLR2 TLR4 TLR7 TLR8 CLEC4E

              炎癥小體成分:CASP1CASP5PYCARDNLRP1

              補(bǔ)體系統(tǒng)成分:C3MBL2MASP1CFBCD46

              GeneQuery qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒,每個(gè)孔包含一個(gè)引物組,可識(shí)別并有效擴(kuò)增特定靶基因的 cDNA。精心設(shè)計(jì)的引物確保:(iqPCR分析中的優(yōu)良退火溫度為65°C(含2 mM Mg2+且不含DMSO;ii)除非另有說(shuō)明,否則引物集可識(shí)別靶基因的所有已知轉(zhuǎn)錄本變體;iii)只有一個(gè)基因被擴(kuò)增。每個(gè)引物組都已通過(guò)qPCR和熔解曲線分析和凝膠電泳進(jìn)行了驗(yàn)證。

              ScienCell's GeneQuery™ Human Innate Immune Response qPCR Array Kit (GQH-IIR) facilitates gene expression profiling of key genes in the human innate immune system.  The innate immune response is activated upon microbial infection or foreign substance presentation and includes activation of the complement system and adaptive immune systems.  A separate array is available for analyzing the adaptive immune response.  Brief examples of how included genes may be grouped according to function are shown below:

              ·         Bacteria response: TLR1, TLR5, NOD1, LY96, TRAM1

              ·         Virus response: TLR3, DDX58, IFIH1, MYD88, IRAK4

              ·         Self recognition: TLR2, TLR4, TLR7, TLR8, CLEC4E

              ·         Inflammasome component: CASP1, CASP5, PYCARD, NLRP1

              ·         Complement system component: C3, MBL2, MASP1, CFB, CD46

              GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

              GeneQuery™ 人類先天免疫應(yīng)答 qPCR 陣列板布局*8 個(gè)粗體和斜體對(duì)照)


              1

              2

              3

              4

              5

              6

              7

              8

              9

              10

              11

              12

              A

              ATF3

              CASP5

              CLEC6A

              IFIH1

              IRAK1

              LYZ

              NAP1L1

              OTUD5

              TICAM1

              TLR5

              TRAF6

              β-actin

              B

              C3

              CD46

              CLEC7A

              IFNA1

              IRAK2

              MAP3K7

              NFAM1

              PYCARD

              TICAM2

              TLR6

              TRAM1

              GAPDH

              C

              C5

              CFB

              DDX58

              IFNB1

              IRAK3

              MAPK8

              NFATC1

              RIPK2

              TIRAP

              TLR7

              TRAM2

              LDHA

              D

              C6

              CFD

              DHX58

              IFNG

              IRAK4

              MASP1

              NFKB1

              RNF125

              TLR1

              TLR8

              TRIM25

              NONO

              E

              C7

              CFH

              EYA4

              IL18

              IRF3

              MASP2

              NFKBIE

              SYK

              TLR10

              TLR9

              TYROBP

              PPIH

              F

              C8A

              CFP

              FADD

              IL1A

              IRF7

              MAVS

              NLRP1

              TAB2

              TLR2

              TNF

              UBE2N

              GDC

              G

              CARD9

              CHUK

              HCST

              IL1B

              JUN

              MBL2

              NOD1

              TAB3

              TLR3

              TRADD

              UBE2V1

              PPC

              H

              CASP1

              CLEC4E

              HMGB1

              IL6

              LY96

              MYD88

              NOD2

              TBK1

              TLR4

              TRAF3

              UNC93B1

              NTC

              提示:基因選擇可能會(huì)根據(jù)新的研究和開(kāi)發(fā)進(jìn)行更新

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